7P3U

Chitin-active fungal AA11 LPMO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.946444
Spacegroup name	P 21 21 21
Unit cell lengths	40.986, 47.283, 115.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.886 - 1.500
Rwork	0.146
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mah
RMSD bond length	0.014
RMSD bond angle	1.827
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.280	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.061	0.361
Number of reflections	36807	1849
<I/σ(I)>	19.2
Completeness [%]	99.8
Redundancy	12.3
CC(1/2)	0.999	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M bicine (pH 9.0) and 20% (w/v) PEG 6000