7P3Q
Streptomyces coelicolor dATP/ATP-loaded NrdR octamer
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.12 |
Software | cryoSPARC,PHENIX,EPU,Coot |
Symmetry | D2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |