7P3B

Human RNA ligase RTCB in complex with GMP and Co(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	96.906, 96.906, 227.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 2.300
R-factor	0.1935
Rwork	0.192
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uc2
RMSD bond length	0.023
RMSD bond angle	1.676
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.068	0.648
Number of reflections	48937	4769
<I/σ(I)>	11.5	1.1
Completeness [%]	99.9	99.4
Redundancy	26.3	25.9
CC(1/2)	0.997	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M sodium acetate pH 5.5, 0.6 M sodium formate, 14% (w/v) PEG 4000, 5 mM cobalt(II) chloride