7P33

Epstein-Barr virus encoded Bcl-2 homolog BHRF-1 in complex with Bid BH3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 65 2 2
Unit cell lengths	94.208, 94.208, 455.582
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.854 - 2.785
R-factor	0.231906605748
Rwork	0.230
R-free	0.27408
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xpx
RMSD bond length	0.002
RMSD bond angle	0.397
Data reduction software	XDS
Data scaling software	Aimless (7.1.007)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.854	2.940
High resolution limit [Å]	2.785	2.785
Rmerge	0.200	1.207
Number of reflections	28893	4267
<I/σ(I)>	7.4
Completeness [%]	92.2
Redundancy	11.9
CC(1/2)	0.997	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.4M Ammonium phosphate mono basic