7P2J
Dimethylated fusion protein of RSL and trimeric coiled coil (4dzn) in complex with cucurbit[7]uril, H3 sheet assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 |
Unit cell lengths | 51.617, 51.617, 322.633 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 107.540 - 1.980 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.24950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bt9 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.540 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmeas | 0.170 | 0.916 |
Rpim | 0.055 | 0.334 |
Number of reflections | 22317 | 1086 |
<I/σ(I)> | 6.8 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.6 | 7.3 |
CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20 % PEG 8000 0.1 M Tris-HCl pH 8.5 0.2 M MgCl2 2 mM Q7 |