7P2I
Dimethylated fusion protein of RSL and nucleoporin peptide (Nup) in complex with cucurbit[7]uril, F432 cage assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 200.713, 200.713, 200.713 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.360 - 1.489 |
R-factor | 0.1624 |
Rwork | 0.162 |
R-free | 0.17790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bt9 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.770 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.10.3 (23-SEP-2019)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.360 | 1.520 |
High resolution limit [Å] | 1.489 | 1.489 |
Rmeas | 0.012 | |
Rpim | 1.400 | |
Number of reflections | 56936 | 2792 |
<I/σ(I)> | 28.7 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 6.9 |
CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25 % PEG 3350 0.1 M Bis-Tris pH 5.5 0.2 M MgCl2 |