7P26
Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT4631_S1_15)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.924, 109.572, 117.531 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.274 - 1.350 |
Rwork | 0.123 |
R-free | 0.13910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7oze |
RMSD bond length | 0.008 |
RMSD bond angle | 1.482 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.570 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 140145 | 6050 |
<I/σ(I)> | 25.4 | 4.5 |
Completeness [%] | 98.8 | |
Redundancy | 12.4 | |
CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 80 mg/ml BT4631 with 10 mM 6S-Gal was crystallised in 20 % PEG 10K and 0.1 M Bicine pH8.5. |