7P22
Thaumatin-like protein of Amycolatopsis rifamycinica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96600 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 80.341, 80.341, 169.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.290 - 1.870 |
R-factor | 0.1828 |
Rwork | 0.181 |
R-free | 0.20650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ahn |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.290 | 1.937 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.132 | 1.127 |
Rmeas | 0.143 | |
Rpim | 0.055 | 0.467 |
Number of reflections | 46839 | 4587 |
<I/σ(I)> | 11.35 | |
Completeness [%] | 99.9 | 99.91 |
Redundancy | 6.6 | 6.7 |
CC(1/2) | 0.997 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.5 | 293 | JCSG+ D1 condition (0.2 M Magnesium chloride hexahydrate, 0.1 M Sodium HEPES pH 7.0 and 30 % v/v PEG 400) |