7P1V
Apo structure of KDNase from Trichophyton Rubrum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2019-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9688 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.030, 180.850, 98.340 |
| Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
| Resolution | 47.360 - 1.470 |
| R-factor | 0.1767 |
| Rwork | 0.174 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.350 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 1.510 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.066 | 0.775 |
| Number of reflections | 276720 | 20438 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium chloride dihydrate 0.1 M MES 6.0 20 % w/v PEG 6000 |
