7P0Q

F(M197)H mutant structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV by fixed-target serial synchrotron crystallography (100K, 26keV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.4769
Spacegroup name	P 42 21 2
Unit cell lengths	99.970, 99.970, 239.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 1.730
R-factor	0.1829
Rwork	0.182
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1j
RMSD bond length	0.012
RMSD bond angle	1.251
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.230
Rpim	0.028
Number of reflections	126837	6215
<I/σ(I)>	17.4	0.5
Completeness [%]	100.0	99.9
Redundancy	68.3	63.6
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO)