7P0H
Crystal structure of Helicobacter pylori ComF fused to an artificial alphaREP crystallization helper(named B2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 |
Unit cell lengths | 57.880, 87.980, 122.790 |
Unit cell angles | 80.23, 76.44, 76.39 |
Refinement procedure
Resolution | 46.470 - 2.499 |
R-factor | 0.2221 |
Rwork | 0.221 |
R-free | 0.24480 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.870 |
Data reduction software | XDS (Nov 11, 2017) |
Data scaling software | STARANISO (1.10.9 (13-Dec-2017)) |
Phasing software | PHASER (2.7.17) |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.470 | 46.410 | 2.560 |
High resolution limit [Å] | 2.490 | 11.150 | 2.490 |
Rmerge | 0.094 | 0.048 | 2.845 |
Rmeas | 0.099 | 0.051 | 2.986 |
Rpim | 0.029 | 0.016 | 0.881 |
Total number of observations | 9133 | 57841 | |
Number of reflections | 77488 | 865 | 5398 |
<I/σ(I)> | 15.7 | 71.3 | 0.6 |
Completeness [%] | 98.4 | 98.3 | 92.6 |
Redundancy | 11.6 | 10.6 | 10.7 |
CC(1/2) | 0.999 | 0.998 | 0.483 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2M Tri-potassium citrate PEG 3350 18% (w/v) |