7P0G
URATE OXIDASE WITH 8-AZAXANTHINE UNDER AMBIENT PRESSURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE CRISTAL |
| Synchrotron site | SOLEIL |
| Beamline | CRISTAL |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-07-28 |
| Detector | RAYONIX SX-165mm |
| Wavelength(s) | 0.58183 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.341, 96.164, 105.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1768 |
| Rwork | 0.174 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1R51 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.808 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.765 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.514 | |
| Number of reflections | 30145 | 4400 |
| <I/σ(I)> | 6.5 | 1.4 |
| Completeness [%] | 93.3 | 94.2 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 20 MG/ML URATE OXIDASE, 8-AZAXANTHINE CONCENTRATION IN EXCESS, 50 MM TRIS/ACETATE, 8% PEG 4000 |
