7P0D

URATE OXIDASE WITH 8-AZAXANTHINE UNDER 310 MPA PRESSURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE CRISTAL
Synchrotron site	SOLEIL
Beamline	CRISTAL
Temperature [K]	293
Detector technology	CCD
Collection date	2019-07-28
Detector	RAYONIX SX-165mm
Wavelength(s)	0.58183
Spacegroup name	I 2 2 2
Unit cell lengths	79.132, 95.398, 104.668
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.192
Rwork	0.188
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 4OP9
RMSD bond length	0.013
RMSD bond angle	1.461
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.506	2.530
High resolution limit [Å]	2.400	2.400
Rmerge		0.635
Number of reflections	15109	2216
<I/σ(I)>	7	1.1
Completeness [%]	96.0	97.2
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	298	20 MG/ML URATE OXIDASE, 8-AZAXANTHINE CONCENTRATION IN EXCESS, 50 MM TRIS/ACETATE, 8% PEG 4000