7P01
Structure of the maltase BaAG2 from Blastobotrys adeninivorans in complex with acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.030, 78.080, 121.930 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 46.320 - 2.120 |
| R-factor | 0.1846 |
| Rwork | 0.184 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aj7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.320 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 140413 | 10323 |
| <I/σ(I)> | 7.68 | 1.54 |
| Completeness [%] | 98.9 | 98.7 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.970 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M MES pH 5.5 12% polyvinylpyrrolidone |
