7OZQ
Crystal structure of archaeal L7Ae bound to eukaryotic kink-loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.391, 61.598, 138.611 |
| Unit cell angles | 90.00, 108.36, 90.00 |
Refinement procedure
| Resolution | 54.500 - 1.910 |
| R-factor | 0.1749 |
| Rwork | 0.173 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18rc4_3812) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.500 | 54.500 | 2.010 |
| High resolution limit [Å] | 1.910 | 6.030 | 1.910 |
| Rmerge | 0.081 | 0.047 | 0.657 |
| Rmeas | 0.088 | 0.051 | 0.713 |
| Rpim | 0.034 | 0.019 | 0.275 |
| Total number of observations | 463836 | 15556 | 65674 |
| Number of reflections | 68501 | 2272 | 9923 |
| <I/σ(I)> | 12.4 | 31.6 | 2.4 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6.8 | 6.8 | 6.6 |
| CC(1/2) | 0.998 | 0.997 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | JCSG Core I, condition G12: 30% MPD, 0.02 M CaCl2, 0.1 M Na acetate |
