7OYY

E.coli's putrescine receptor variant PotF/D (4JDF) with mutation S247D in complex with spermidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	37.059, 82.146, 111.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.150 - 1.360
R-factor	0.1571
Rwork	0.156
R-free	0.18590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a99
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	XDS (20190806)
Data scaling software	XSCALE (20190806)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.150	1.410
High resolution limit [Å]	1.360	1.360
Rmerge	0.171	2.530
Rmeas	0.184	2.721
Rpim	0.068	0.988
Number of reflections	73425	52254
<I/σ(I)>	7.58	0.61
Completeness [%]	99.5	98
Redundancy	7.3	7.3
CC(1/2)	0.997	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M MES pH 5, 30% PEG 6000