7OYW
E.coli's putrescine receptor variant PotF/D (4JDF) with mutations E39D F88L S247D in complex with spermidine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 116.881, 71.285, 92.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.390 - 1.280 |
R-factor | 0.1327 |
Rwork | 0.132 |
R-free | 0.16130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a99 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.045 |
Data reduction software | XDS (20190806) |
Data scaling software | XSCALE (20190806) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.390 | 1.330 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.104 | 2.316 |
Rmeas | 0.114 | 2.536 |
Rpim | 0.044 | 1.015 |
Number of reflections | 198363 | 19073 |
<I/σ(I)> | 8.8 | 0.59 |
Completeness [%] | 99.3 | 96.4 |
Redundancy | 6.6 | 6 |
CC(1/2) | 0.997 | 0.233 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 2.4 M AmSO4 + 0.1 M Bicine pH 9.0 + 5% Jeffamine M600 |