7OYK
DNA-binding domain of CggR in complex with the DNA operator
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.972, 103.141, 51.111 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 45.837 - 2.101 |
| Rwork | 0.208 |
| R-free | 0.25270 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.336 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.086 | 1.111 |
| Number of reflections | 34417 | 10673 |
| <I/σ(I)> | 9.4 | 1.1 |
| Completeness [%] | 96.8 | 95.4 |
| Redundancy | 2.3 | 2.3 |
| CC(1/2) | 0.998 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 10% (w/v) PEG 3350, 100mM MES, pH 6.5, 100mM calcium chloride, 13% (v/v) glycerol |
