7OYI
Escherichia coli YtfE_E159L(MN)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-07-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.976247 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.824, 49.671, 88.091 |
Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
Resolution | 53.390 - 1.860 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.20470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fnn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.790 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.390 | 1.880 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.048 | |
Rmeas | 0.058 | |
Rpim | 0.031 | |
Number of reflections | 43000 | 2139 |
<I/σ(I)> | 13 | |
Completeness [%] | 99.3 | |
Redundancy | 3.3 | |
CC(1/2) | 0.990 | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | Tris-HCl pH 8.5, PEG 4000, magnesium chloride, manganese chloride |