7OXX
CrabP2 mutant R30AK31A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.170, 106.020, 55.540 |
| Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
| Resolution | 54.386 - 1.330 |
| Rwork | 0.158 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2frs |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.859 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.390 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Number of reflections | 128510 | 6247 |
| <I/σ(I)> | 9.7 | 1.4 |
| Completeness [%] | 97.1 | 94.7 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.996 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 150 mM MgCl2, 100 mM Bis-Tris pH 6.5 and 25% PEG 3350. |
