7OXV
Crystal structure of depupylase Dop in the Dop-loop-inserted state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.533, 72.533, 184.149 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.037 - 1.394 |
R-factor | 0.1353 |
Rwork | 0.134 |
R-free | 0.16920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lrt |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.040 | 1.444 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 98553 | 9612 |
<I/σ(I)> | 26.76 | |
Completeness [%] | 99.8 | 98.99 |
Redundancy | 25.2 | 22.6 |
CC(1/2) | 1.000 | 0.587 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 14-20 % (w/v) PEG 3350, 100 mM Bis-Tris propane pH 6.0-6.5 and 100-200 mM potassium thiocyanate (KSCN) |