7OXS
1.91 A crystal structure of DNA/2'-O-methyl-RNA heteroduplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-02 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.91840 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 56.130, 56.130, 76.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.420 - 1.910 |
R-factor | 0.1848 |
Rwork | 0.184 |
R-free | 0.19540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ow0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.109 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.610 | 2.020 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmeas | 0.033 | 1.063 |
Number of reflections | 20931 | 3348 |
<I/σ(I)> | 31.56 | |
Completeness [%] | 99.6 | 97.9 |
Redundancy | 10.3 | 2.01 |
CC(1/2) | 1.000 | 0.757 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0 M Li2SO4, 50 mM sodium cacodylate pH 6.5, 10 mM spermine, 15 mM MgCl2 |