7OXD
Crystal structure of Scytonema hofmanni transposition protein TniQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.636, 49.875, 86.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.240 - 1.300 |
R-factor | 0.1889 |
Rwork | 0.188 |
R-free | 0.19520 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.400 | 1.346 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.046 | 0.964 |
Rmeas | 0.047 | |
Rpim | 0.013 | 0.285 |
Number of reflections | 85839 | 53615 |
<I/σ(I)> | 32.11 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.7 | 12.1 |
CC(1/2) | 1.000 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 280 | 100 mM HEPES pH 7.5 20% PEG 6000 250 mM sodium chloride |