7OXA
Target-bound SpCas9 complex with AAVS1 chimeric RNA-DNA guide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.926, 67.574, 189.232 |
Unit cell angles | 90.00, 112.52, 90.00 |
Refinement procedure
Resolution | 47.660 - 2.150 |
R-factor | 0.1991 |
Rwork | 0.198 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fq5 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.618 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.660 | 2.227 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.113 | 2.230 |
Number of reflections | 113319 | 11255 |
<I/σ(I)> | 12.36 | 0.78 |
Completeness [%] | 99.9 | 99.81 |
Redundancy | 6.8 | 6.7 |
CC(1/2) | 0.999 | 0.282 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Tris-acetate pH 8.5, 0.3-0.5 M KSCN, 17-19% PEG3350 |