7OX3

Fab 6D3: hIL-9 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	60.480, 81.860, 116.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.640 - 1.700
R-factor	0.1781
Rwork	0.176
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5niv 6ct7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	63819	10037
<I/σ(I)>	21.59
Completeness [%]	99.2
Redundancy	13.4
CC(1/2)	0.999	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	0.1M ammonium sulfate, 0.1M Tris, 16% PEG 1500, pH 5.8