7OWN
HsNMT1 in complex with both MyrCoA and peptide AKSFSKPR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98012 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 79.689, 178.442, 58.248 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.770 - 2.100 |
R-factor | 0.1689 |
Rwork | 0.166 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sk2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.913 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.770 | 2.060 |
High resolution limit [Å] | 2.010 | 2.010 |
Rpim | 0.046 | 0.428 |
Number of reflections | 55446 | 3702 |
<I/σ(I)> | 15.9 | 2 |
Completeness [%] | 99.4 | 92.1 |
Redundancy | 14.9 | 13 |
CC(1/2) | 0.999 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 22% PEG6K, 100mM Sodium Citrate pH 5.6, 100mM MgCl2, 100mM NaCl |