7OWJ

Odinarchaeota Adenylate kinase (OdinAK) in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	77.608, 78.787, 217.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.270 - 2.500
R-factor	0.1904
Rwork	0.187
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7owe
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.270	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.142	0.880
Rpim	0.088	0.548
Number of reflections	46830	4562
<I/σ(I)>	8.2	2
Completeness [%]	99.7	98.5
Redundancy	6.8
CC(1/2)	0.994	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	Protein in: 0.030 M MOPS, 0.050M NaCl, pH 6.8. Drop: 1:1 of Bis-Tris Propane pH 6.5 - 0.1 M, PEG 3350 12% Odin 0.853 mM, GTP 8.53 mM