7OW0
1.55 A crystal structure of DNA/2'-O-methyl-RNA heteroduplex with overhangs solved by Zn-SAD.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.28308 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 31.850, 31.850, 91.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.090 - 1.550 |
R-factor | 0.2241 |
Rwork | 0.221 |
R-free | 0.25080 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.624 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXD |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.850 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.049 | 0.586 |
Rmeas | 0.053 | 0.696 |
Number of reflections | 12966 | 1979 |
<I/σ(I)> | 18.92 | 1.96 |
Completeness [%] | 99.0 | 94.6 |
Redundancy | 6.03 | 3.36 |
CC(1/2) | 0.998 | 0.771 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.8 M Li2SO4, 50 mM Na cacodylate pH 6.5, 5 mM spermine, 50 mM ZnCl2 |