7OVP
Crystal structure of the chemotactic adaptor protein CheF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.620, 188.600, 58.290 |
| Unit cell angles | 90.00, 112.90, 90.00 |
Refinement procedure
| Resolution | 46.630 - 2.900 |
| R-factor | 0.2398 |
| Rwork | 0.237 |
| R-free | 0.28440 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.021 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.630 | 2.840 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 26066 | 2567 |
| <I/σ(I)> | 23.1 | 1.04 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.6 | |
| CC(1/2) | 0.999 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Tris-HCl pH 7.8, 0.1 M ammonium sulfate, 0.3 M sodium formate, 3% PGA-LM, 3% PEG 8000 |
