7OV0
Orotidine 5'-monophosphate decarboxylase-domain of human UMPS in resting state at 0.95 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.7653 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.396, 116.562, 61.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.450 - 0.950 |
R-factor | 0.1056 |
Rwork | 0.105 |
R-free | 0.11750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qcd |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.450 | 42.450 | 0.970 |
High resolution limit [Å] | 0.950 | 4.250 | 0.950 |
Rmerge | 0.033 | 0.022 | 0.639 |
Rmeas | 0.037 | 0.025 | 0.760 |
Total number of observations | 736718 | ||
Number of reflections | 172156 | 1899 | 12806 |
<I/σ(I)> | 18.05 | 50.05 | 1.78 |
Completeness [%] | 98.2 | 88.8 | 99.4 |
Redundancy | 4.279 | 4.247 | 3.316 |
CC(1/2) | 0.999 | 0.998 | 0.681 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293.15 | Ammonium sulfate, Tris/HCl, Glutathion, Glycerol |