7OUU

Crystal structure of human filamin C domains 14-15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	45.040, 58.820, 178.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 1.470
R-factor	0.1894
Rwork	0.188
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	morda
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MoRDa
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.830	1.523
High resolution limit [Å]	1.470	1.470
Rmerge	0.114	9.860
Rmeas	0.119	10.840
Rpim	0.034	4.376
Number of reflections	71856	3680
<I/σ(I)>	11.1	0.11
Completeness [%]	88.0	45.84
Redundancy	11	5.7
CC(1/2)	0.999	0.019

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295.15	0.1 M HEPES 7.0, 30 % v/v Jeffamine ED-2003