7OUQ
wilavidin biotin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976252 |
Spacegroup name | I 21 3 |
Unit cell lengths | 180.132, 180.132, 180.132 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.030 - 2.630 |
R-factor | 0.1794 |
Rwork | 0.178 |
R-free | 0.20020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3szj |
RMSD bond length | 0.011 |
RMSD bond angle | 2.073 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.030 | 2.770 |
High resolution limit [Å] | 2.630 | 2.630 |
Number of reflections | 28877 | 2810 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 99.8 | |
Redundancy | 21.6 | |
CC(1/2) | 0.999 | 0.392 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8 M Ammonium sulfate |