7OUJ
Crystal structure of the flavoprotein monooxygenase RubL from rubromycin biosynthesis
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 77.323, 57.514, 122.379 |
Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
Resolution | 37.778 - 1.573 |
Rwork | 0.183 |
R-free | 0.20950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ihg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.702 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.080 | 1.660 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.046 | 0.550 |
Number of reflections | 70014 | 10481 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 94.0 | |
Redundancy | 2.4 | |
CC(1/2) | 0.990 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20 mM DL-Arginine hydrochloride, 20 mM DL-Threonine, 20 mM DL-Histidine monohydrochloride monohydrate, 20 mM DL-5-Hydroxylysine hydrochloride, 20 mM trans-4-hydroxy-L-proline, 100 mM BES/TEA pH 7.5 buffer system, 12.5% PEG 4000 and 20% 1,2,6-Hexanetriol |