7OUC
Crystal structure of the flavoprotein monooxygenase GrhO5 from griseorhodin A biosynthesis
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.069, 104.437, 69.226 |
Unit cell angles | 90.00, 104.89, 90.00 |
Refinement procedure
Resolution | 44.520 - 1.750 |
Rwork | 0.194 |
R-free | 0.23270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ihg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.594 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.563 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.150 | 0.630 |
Rpim | 0.090 | |
Number of reflections | 62285 | 8665 |
<I/σ(I)> | 5 | |
Completeness [%] | 97.8 | |
Redundancy | 3.2 | |
CC(1/2) | 0.980 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20 % PEG 3350, 200 mM di-Sodium malonate |