7OU4
The structure of MutS bound to one molecule of ATP and one molecule of ADP
EM specimen
| Vitrification | Yes |
| Instrument | LEICA PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.3 |
| Number of Particle | 527979 |
| Software | RELION,REFMAC,EPU,Gctf,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
