7OU3

Crystal structure of Tga-AGOG, an 8-oxoguanine DNA glycosylase from Thermococcus gammatolerans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	61.429, 62.452, 139.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.150 - 1.490
R-factor	0.1732
Rwork	0.172
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xqo
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.940	1.520
High resolution limit [Å]	1.490	1.490
Rmerge	0.071	0.844
Number of reflections	88713	4393
<I/σ(I)>	13.6	2.3
Completeness [%]	100.0	100
Redundancy	7.3	7.3
CC(1/2)	0.998	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	Hepes, NaCl, PEG 1500, glycerol