7OTR
Crystal structure of a psychrophilic CCA-adding enzyme determined by SAD phasing
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-28 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 2.0751 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.790, 69.790, 291.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.350 - 2.250 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.20780 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.964 |
Data reduction software | XDS (20200417) |
Data scaling software | XSCALE (20200417) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.310 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmeas | 0.170 | 3.110 |
Number of reflections | 64470 | 4607 |
<I/σ(I)> | 30.7 | |
Completeness [%] | 99.0 | 96.2 |
Redundancy | 78 | 25 |
CC(1/2) | 0.999 | 0.425 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein solution at 4.5 mg/mL in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 5 mM MgCl2; reservoir solution: 100 mM Sodium acetate; pH 4.5 1 M di-Ammonium hydrogen phosphate |