7OTL
Structure of a psychrophilic CCA-adding enzyme crystallized by counter-diffusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-26 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.000, 70.000, 292.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.880 - 2.800 |
R-factor | 0.2243 |
Rwork | 0.223 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qxn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmeas | 0.258 | 2.040 |
Number of reflections | 18615 | 1819 |
<I/σ(I)> | 21.3 | 3.1 |
Completeness [%] | 98.6 | 100 |
Redundancy | 55.7 | 57.5 |
CC(1/2) | 0.999 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 5 | 293 | Protein solution at 4.5 mg/mL in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 5 mM MgCl2; Crystals were obtained in capillaries filled with protein solution and sealed at one extremity and plunged in 3 M ammonium sulfate, 0.1 M sodium acetate pH 5. |