7OTJ

Crystal structure of Pif1 helicase from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	C 1 2 1
Unit cell lengths	198.973, 79.960, 94.110
Unit cell angles	90.00, 117.28, 90.00

Refinement procedure

Resolution	82.510 - 2.580
R-factor	0.2081
Rwork	0.206
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5o6b
RMSD bond length	0.004
RMSD bond angle	0.710
Data reduction software	XDS (built on 20170615)
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.510	2.620
High resolution limit [Å]	2.575	2.575
Rmerge	0.089	0.447
Rmeas	0.106	0.527
Rpim	0.058	0.278
Number of reflections	40378	2065
<I/σ(I)>	8.6	2.1
Completeness [%]	96.7	99.8
Redundancy	3.4	3.5
CC(1/2)	0.992	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Tris-HCl 0.1M pH 7.5 PEG 4K 20%