7OSM

Intermediate translocation complex of 80 S.cerevisiae ribosome with eEF2 and ligands

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	233.490, 299.850, 513.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	149.930 - 3.000
R-factor	0.2278
Rwork	0.228
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v88
RMSD bond length	0.005
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	149.930	3.314
High resolution limit [Å]	3.000	3.200
Number of reflections	712272	58317
<I/σ(I)>	13.35	0.94
Completeness [%]	100.0
Redundancy	172
CC(1/2)	0.990	0.140

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 6000, KSCN, Tris-HAc