7OS7
Circular permutant of ribosomal protein S6, swap helix 2, L75A, A92K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97996 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.646, 55.646, 148.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.110 - 1.650 |
| R-factor | 0.1647 |
| Rwork | 0.161 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ris |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.696 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 148.560 | 148.560 | 1.670 |
| High resolution limit [Å] | 1.650 | 8.860 | 1.650 |
| Rmerge | 0.067 | 0.052 | 1.288 |
| Rmeas | 0.072 | 0.057 | 1.367 |
| Rpim | 0.024 | 0.022 | 0.450 |
| Total number of observations | 253548 | 1430 | 12880 |
| Number of reflections | 29194 | 239 | 1438 |
| <I/σ(I)> | 15.9 | 33.9 | 2 |
| Completeness [%] | 99.7 | 95.9 | 98.8 |
| Redundancy | 8.7 | 6 | 9 |
| CC(1/2) | 0.998 | 0.993 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium citrate dibasic 14% w/v PEG 3350 |
