7OS6
Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP1)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.812, 91.044, 113.662 |
| Unit cell angles | 90.00, 97.08, 90.00 |
Refinement procedure
| Resolution | 77.220 - 1.430 |
| Rwork | 0.159 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | XXXX |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.681 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.220 | 1.520 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.073 | 0.818 |
| Rmeas | 0.084 | 0.952 |
| Number of reflections | 287575 | 45258 |
| <I/σ(I)> | 10.2 | 1.5 |
| Completeness [%] | 99.3 | 96.8 |
| Redundancy | 4.1 | 3.4 |
| CC(1/2) | 0.998 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 25% PEG3350, 0.2 M Li2SO4, 0.1 M Tris pH 8.0, 20 mM n-octyl-beta-D-thioglucoside |
