7OS0
Structure of the Rhodobacter capsulatus Cas13a-crRNA binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-09-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.387, 91.082, 136.509 |
| Unit cell angles | 89.99, 103.69, 97.65 |
Refinement procedure
| Resolution | 47.120 - 2.200 |
| R-factor | 0.2202 |
| Rwork | 0.218 |
| R-free | 0.25570 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS (VERSION Jan 26, 2018) |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.085 | 0.913 |
| Number of reflections | 141242 | 14010 |
| <I/σ(I)> | 9.6 | 1.2 |
| Completeness [%] | 97.6 | 96.3 |
| Redundancy | 3.6 | 3.4 |
| CC(1/2) | 0.997 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.0, 200 mM NaCl and 20 % (w/v) PEG6000 |
