7ORT
Ternary complex of 14-3-3 sigma, p27pT198 phosphopeptide, and WQ176
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-22 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54187 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.579, 112.025, 62.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.750 - 2.330 |
R-factor | 0.1792 |
Rwork | 0.177 |
R-free | 0.22830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc3 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.750 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.750 | 2.370 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.053 | 0.081 |
Number of reflections | 12594 | 539 |
<I/σ(I)> | 23.8 | 17.2 |
Completeness [%] | 99.0 | 87.8 |
Redundancy | 6.1 | 5.5 |
CC(1/2) | 0.997 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.095 M Hepes pH7.3, 26%PEG 400, 0.19 M CaCl2, and 5 % Glycerol |