7ORD
The crystal structure of the domain-swapped dimer of onconase (2)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.750, 28.540, 61.480 |
Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
Resolution | 58.590 - 2.140 |
Rwork | 0.212 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1onc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.636 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.590 | 2.180 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmeas | 0.124 | |
Number of reflections | 11313 | 11313 |
<I/σ(I)> | 10.6 | 2.3 |
Completeness [%] | 97.4 | |
Redundancy | 5.9 | 4.3 |
CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris-HCl pH 8.5 25% PEG 3350 0.2 M Ammonium acetate |