7OQX
Crystal structure of a psychrophilic CCA-adding enzyme in complex with CMPcPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.800, 69.800, 290.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.740 - 2.200 |
R-factor | 0.2073 |
Rwork | 0.205 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qxn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.930 |
Data reduction software | XDS (20200417) |
Data scaling software | XSCALE (20200417) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.150 | 1.616 |
Number of reflections | 37711 | 5903 |
<I/σ(I)> | 16.84 | 1.98 |
Completeness [%] | 99.8 | 99.3 |
Redundancy | 25.7 | 27.1 |
CC(1/2) | 0.990 | 0.786 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | protein solution at 4.5 mg/mL in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 5 mM MgCl2; reservoir solution: 100 mM Sodium acetate; pH 4.5 1 M di-Ammonium hydrogen phosphate remarks : soaking 30 seconds in a drop with 10 mM CMPcPP and reservoir solution + 20% glycerol |