7OQV
Crystal structure of the polymerising VEL domain of VIN3 (I575D mutant)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.498, 91.273, 98.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.070 - 2.400 |
Rwork | 0.223 |
R-free | 0.26950 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.532 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.070 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 11740 | 11740 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 100.0 | |
Redundancy | 8.7 | |
CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 1M potassium phosphate monobasic, 3 % v/v 2 propanol, 0.1M sodium cacodylate pH6.5 |