7OQG
Ternary complex of 14-3-3 sigma, Amot-p130 phosphopeptide, and WQ136
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97629 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.810, 111.321, 61.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.350 - 1.500 |
| R-factor | 0.1605 |
| Rwork | 0.160 |
| R-free | 0.17830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jc3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.322 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.350 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.082 | 0.723 |
| Number of reflections | 45787 | 2225 |
| <I/σ(I)> | 16.3 | 3.2 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 12 | 12.3 |
| CC(1/2) | 0.998 | 0.616 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.095 M Hepes pH 7.5, 26% PEG 400, 0.19 M CaCl2, and 5% Glycerol |
