7OQE
Saccharomyces cerevisiae spliceosomal pre-A complex (delta BS-A ACT1)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.9 |
| Software | RELION |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
