7OPK

Crystal structure of C. thermophilum Xrn2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-02
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	198.780, 198.780, 69.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.690 - 3.000
R-factor	0.1822
Rwork	0.180
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fqd
RMSD bond length	0.003
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.107
High resolution limit [Å]	3.000	3.000
Rmeas	0.308	2.037
Rpim	0.068	0.455
Number of reflections	31644	3130
<I/σ(I)>	13.65	1.89
Completeness [%]	99.9	99.94
Redundancy	20	19.9
CC(1/2)	0.996	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Magnesium formate, 20 %(w/v) PEG 3350